Venom component allergen IgE measurement in the diagnosis and management of insect sting allergy

Simon Blank^*, Peter Korošec, Benjamin O. Slusarenko, Markus Ollert, Robert G. Hamilton^*

Journal of Allergy and Clinical Immunollogy: In Practise, Online ahead of print (2024)

Basophil activation test in Hymenoptera venom allergy

Bernadette Eberlein, Knut Brockow, Ulf Darsow, Tilo Biedermann, Simon Blank

Allergologie Select, 8:293-298 (2023) (2024)

Activation of the aryl hydrocarbon receptor improves allergen-specific immunotherapy of murine allergic airway inflammation: A novel adjuvant option?

Sonja Heine, Francesca Alessandrini, Johannes Grosch, Carina Graß, Alexander Heldner, Benjamin Schnautz, Johanna Grosch, Jeroen Buters, Benjamin O. Slusarenko, Daniel Krappmann, Francesca Fallarino, Caspar Ohnmacht, Carsten B. Schmidt-Weber, Simon Blank

Frontiers in Immunology, 15:1397072. (2024)

Basophil activation in insect venom allergy: Comparison of an established test using liquid reagents with a test using 5-color tubes with dried antibody reagents

Sebastian Waldherr, Miriam Hils, Martin Köberle, Knut Brockow, Ulf Darsow, Simon Blank, Tilo Biedermann, Bernadette Eberlein

BMC Immunology, 25(1):23 (2024)

Animal toxins: biodiscovery, mechanistic insights and translational potential

Tim Lüddecke & Simon Blank

Toxins, 16(3):130 (2024)

Basophil activation test (BAT) in double-sensitized patients with Hymenoptera venom allergy: additional benefit of component-resolved diagnostics

Paul Schmidle, Simon Blank, Sabine Altrichter, Wolfram Hötzenecker, Knut Brockow, Ulf Darsow, Tilo Biedermann, Bernadette Eberlein

Journal of Allergy and Clinical Immunollogy: In Practise, 11(9):2890-2899(2023)

EAACI Molecular Allergology User's Guide 2.0

Stephanie Dramburg, Christiane Hilger, Alexandra Santos, Leticia de las Vecillas, Rob C. Aalberse, Nathalie Acevedo, Lorenz Aglas, Friedrich Altmann, Karla L. Arruda, Riccardo Asero, Barbara Ballmer-Weber, Domingo Barber, Kirsten Beyer, Tilo Biedermann, Beatrice M. Bilo, Simon Blank, Philipp P. Bosshard, Heimo Breiteneder, Helen A. Brough, Merima Bublin, Dianne Campbell, Luis Caraballo, Jean Christoph Caubet, Giorgio Celi, Martin D. Chapman, Maksymilian Chruszcz, Adnan Custovic, Rebecca Czolk, Janet Davies, Nikolaos Douladiris, Bernadette Eberlein, Motohiro Ebisawa, Anna Ehlers, Philippe Eigenmann, Gabriele Gadermaier, Mattia Giovannini, Francisca Gomez, Rebecca Grohman, Carole Guillet, Christine Hafner, Robert G. Hamilton, Michael Hauser, Thomas Hawranek, Hans Jürgen Hoffmann, Thomas Holzhauser, Tomona Iizuka, Alain Jacquet, Thilo Jakob, Bente Janssen-Weets, Uta Jappe, Marek Jutel, Tanja Kalic, Sandip Kamath, Sabine Kespohl, Jörg Kleine-Tebbe, Edward Knol, André Knulst, Jon R. Konradsen, Peter Korošec, Annette Kuehn, Gideon Lack, Thuy-My Le, Andreas Lopata, Olga Luengo, Mika Mäkelä, Alessandro Maria Marra, Martine Morisset, Antonella Muraro, Anna Nowak-Wegrzyn, Roni Nugraha, Markus Ollert, Kati Palosuo, Elide Anna Pastorello, Sarita Ulhas Patil, Thomas Platts-Mills, Anna Pomés, Pascal Poncet, Ekaterina Potapova, Lars K. Poulsen, Christian Radauer, Suzana Radulovic, Monika Raulf, Pierre Rougé, Joaquin Sastre, Sakura Sato, Enrico Scala, Johannes M. Schmid, Peter Schmid-Grendelmeier, Denise Schrama, Hélène Sénéchal, Claudia Traidl-Hoffmann, Marcela Valverde-Monge, Marianne van Hage, Ronald van Ree, Kitty Verhoeckx, Stefan Vieths, Magnus Wickman, Josefina Zakzuk, Paolo M. Matricardi, Karin Hoffmann-Sommergruber

Pediatric Allergy and Immunology, 34 Suppl 28:e13854 (2023)

Ex vivo immunomodulatory effects of Lactobacillus-, Lacticaseibacillus- and Bifidobacterium-containing synbiotics on human peripheral blood mononuclear cells and monocyte-derived dendritic cells in the context of grass pollen allergy

Alexander Heldner, Matthew D. Heath, Benjamin Schnautz, Sebastian Kotz, Adam Chaker, Matthias F. Kramer, Constanze A. Jakwerth, Ulrich M. Zissler, Carsten B. Schmidt-Weber, Simon Blank

Probiotics and Antimicrobial Proteins, 15(4):868-879 (2023)

Molecular determination of insect venom allergies

Simon Blank, Constanze A. Jakwerth, Ulrich M. Zissler, Carsten B. Schmidt-Weber

Expert Reviews of Molecular Diagnostics, 22(11):983-986 (2022)

Role of microRNAs in Type 2 Diseases and Allergen-specific Immunotherapy

Constanze A. Jakwerth, Hannah Kitzberger, Dimitrii Pogorelov, Annika Müller, Simon Blank, Carsten B. Schmidt-Weber, Ulrich M. Zissler

Frontiers in Allergy, 3:993937 (2022)

PLGA-PEG-PLGA hydrogel as depot matrix for allergen-specific immunotherapy

Sonja Heine, Antonio Aguilar-Pimentel, Dennis Russkamp, Francesca Alessandrini, Valerie Gailus-Durner, Helmut Fuchs, Markus Ollert, Reinhard Bredehorst, Caspar Ohnmacht, Ulrich M. Zissler, Martin Hrabě de Angelis, Carsten B. Schmidt-Weber, Simon Blank

Pharmaceutics, 14(8):1527 (2022)

A novel monoclonal IgG1 antibody specific for galactose-alpha-1,3-galactose questions alpha-gal epitope expression by bacteria

Luisa Kreft, Aloys Schepers, Miriam Hils, Kyra Swiontek, Andrew Flatley, Robert Janowski, Mohammadali Khan Mirzaei, Michael Dittmar, Neera Chakrapani, Mahesh S. Desai, Stefanie Eyerich, Li Deng, Dierk Niessing, Konrad Fischer, Regina Feederle, Simon Blank , Carsten B. Schmidt-Weber, Christiane Hilger, Tilo Biedermann, Caspar Ohnmacht

Frontiers in Immunology, 13:958952 (2022)

Allergen content of therapeutic preparations for allergen-specific immunotherapy of European paper wasp venom allergy

Johannes Grosch, Antoine Lesur, Stéphanie Kler, François Bernardin, Gunnar Dittmar, Elisabetta Francescato, Simon J. Hewings, Constanze A. Jakwerth, Ulrich M. Zissler, Matthew D. Heath, Markus Ollert, Matthias F. Kramer, Christiane Hilger, Maria Beatrice Bilò, Carsten B. Schmidt-Weber, Simon Blank

Toxins, 14(4):284 (2022)

Role of respiratory epithelial cells in allergic diseases

Constanze A. Jakwerth, José Ordovas-Montanes, Simon Blank, Carsten B. Schmidt-Weber, Ulrich M. Zissler

Cells,11(9):1387 (2022)

α-Gal present on both glycolipids and glycoproteins contributes to immune response in meat-allergic patients

Neera Chakrapani, Jörg Fischer, Kyra Swiontek, Françoise Codreanu-Morel, Farah Hannachi, Martine Morisset, Clément Mugemana, Dmitry Bulaev, Simon Blank, Carsten Bindslev-Jensen, Tilo Biedermann, Markus Ollert, Christiane Hilger

Journal of Allergy and Clinical Immunology, 150(2):396-405 (2022)

Venom Immunotherapy: From proteins to product to patient’s protection

Martin Feindor, Matthew D Heath, Simon J Hewings, Thalia L Carreno Velazquez, Simon Blank, Johannes Grosch, Thilo Jakob, Peter Schmid-Grendelmeier, Ludger Klimek, David BK Golden, Murray A Skinner, Matthias F Kramer

Toxins, 13(9):616 (2021)

Characterization of new allergens from the venom of the European paper wasp Polistes dominula

Johannes Grosch, Bernadette Eberlein, Sebastian Waldherr, Mariona Pascal, Clara San Bartolome, Federico De La Roca Pinzón, Michael Dittmar, Christiane Hilger, Markus Ollert, Tilo Biedermann, Ulf Darsow, Maria Beatrice Bilò, Carsten B. Schmidt-Weber, Simon Blank

Toxins, 13(8):559 (2021)

Immunological effects of adjuvanted low-dose allergoid allergen-specific immunotherapy in experimental murine house dust mite allergy

Alexander Heldner, Francesca Alessandrini, Dennis Russkamp, Sonja Heine, Benjamin Schnautz, Adam Chaker, Juliet Mwange, Thalia L. Carreno Velazquez, Matthew D. Heath, Murray A. Skinner, Matthias F. Kramer, Ulrich M. Zissler, Carsten B. Schmidt-Weber, Simon Blank

Allergy, 77(3):907-919 (2021)

An exhausted phenotype of Th2-Cells is primed by allergen exposure, but not reinforced by allergen-specific immunotherapy

Shu-Hung Wang*, Ulrich M. Zissler*, Maren Buettner, Sonja Heine, Alexander Heldner, Sebastian Kotz, Lisa Pechtold, Jopsephine Kau, Mirjam Plaschke, Julia Schilling, Ferdinand Guerth, Madlen Oelsner, Francesca Alessandrini, Simon Blank, Adam M. Chaker, Carsten B. Schmidt-Weber, Constanze A. Jakwerth

Allergy, 76(9):2827-2839 (2021)

Editorial: Novel Advances in Allergy Diagnosis and Treatment

Simon Blank and Christiane Hilger

Frontiers in Immunology, 12:662699 (2021)

Comprehensive mapping of immune tolerance yields a regulatory TNF receptor 2 signature in a murine model of successful Fel d 1-specific immunotherapy using high-dose CpG adjuvant

Cathy Leonard, Guillem Montamat, Caroline Davril, Olivia Domingues, Oliver Hunewald, Dominique Revets, Coralie Guerin, Simon Blank, Justine Heckendorn, Gauthier Jardon, François Hentges, Markus Ollert

Allergy, 76(7):2153-2165 (2021)

Component-based basophil activation test as a decisive in vitro test to detect sensitization in cases of insect venom allergy

Bernadette Eberlein, Paul Schmidle, Simon Blank, Felix King, Tilo Biedermann, Ulf Darsow, Knut Brockow

Allergologie, 44(8):615-618 (2021)

Marker allergens in Hymenoptera venom allergy - Characteristics and potential use in precision medicine

Simon Blank, Maria Beatrice Bilò, Johannes Grosch, Carsten B. Schmidt-Weber, Markus Ollert, Thilo Jakob

Allergo Journal International, 30:26-38 (2021)

Precision medicine in Hymenoptera venom allergy: Diagnostics, biomarkers and therapy of different endotypes and phenotypes

Simon Blank*, Johannes Grosch, Markus Ollert, Maria Beatrice Bilò*

Frontiers in Immunology, 11:579409 (2020)

Antigen 5 allergens of Hymenoptera venoms and their role in diagnosis and therapy of venom allergy

Simon Blank*, Murilo Luiz Bazon, Johannes Grosch, Carsten B. Schmidt-Weber, Márcia Regina Brochetto-Braga, Maria Beatrice Bilò, Thilo Jakob*

Current Allergy and Asthma Reports, 20(10):58 (2020)

The honeybee venom major allergen Api m 10 (icarapin) and its role in diagnostics and treatment of Hymenoptera venom allergy

Thilo Jakob*, Michèle Myriam Rauber, Amilcar Perez-Riverol, Edzard Spillner, Simon Blank*

Current Allergy and Asthma Reports, 20(9):48 (2020)

Standard methods for Apis mellifera venom research

Dirk C. de Graaf*, Márcia Regina Brochetto Braga*, Rusleyd Maria Magalhães de Abreu, Simon Blank, Chris H. Bridts, Luc S. De Clerck, Bart Devreese, Didier G. Ebo, Timothy J. Ferris, Margo M. Hagendorens, Débora Laís Justo Jacomini, Iliya Kanchev, Zenon J. Kokot, Jan Matysiak, Christel Mertens, Vito Sabato, Athina L. Van Gasse, Matthias Van Vaerenbergh. In V. Dietemann, P. Neumann, N. L. Carreck, & J. D. Ellis (Eds.), The COLOSS BEEBOOK – Volume III, Part 2: Standard methods for Apis mellifera product research

Journal of Apicultural Research (2021)

Shedding light on the venom proteomes of the allergy-relevant Hymenoptera Polistes dominula (European paper wasp) and Vespula spp. (yellow jacket)

Johannes Grosch, Christiane Hilger, Maria Beatrice Bilò, Stephanie Kler, Maximilian Schiener, Gunnar Dittmar, François Bernardin, Antoine Lesur, Markus Ollert, Carsten B. Schmidt-Weber, Simon Blank

Toxins, 12(5):E323 (2020)

Insektengiftallergie: Komponentenaufgelöste Diagnostik und ihre Implikationen für die Therapie

Simon Blank

Schweizer Zeitschrift für Dermatologie, 03/20:4-8 (2020)

Fatal anaphylaxis following a hornet sting in a yellow jacket venom-sensitized patient with undetected monoclonal mast cell activation syndrome and without previous history of a systemic sting reaction

Simon Blank, Sinan Pehlivanli, Heiko Methe, Carsten B Schmidt-Weber, Tilo Biedermann, Hans-Peter Horny, Thomas Kristensen, Yacine Amar, Martin Köberle, Knut Brockow, Peter E Stömmer

Journal of Allergy and Clinical Immunollogy: In Practise, 8(1):401-403 (2020)

The role of mobile health technologies in allergy care: an EAACI position paper

Paolo Maria Matricardi, Stephanie Dramburg, Alberto Álvarez-Perea, Darío Antolín-Amérigo, Christian Apfelbacher, Marina Atanaskovic-Markovic, Uwe Berger, Michael S Blaiss, Simon Blank, Elisa Boni, Matteo Bonini, Jean Bousquet, Knut Brockow, Jeroen Buters, Victoria Cardona, Jean-Christoph Caubet, Özlem Cavkaytar, Tania Elliott, Ignacio Esteban-Gorgojo, Joao A. Fonseca, James Gardner, Philippe Gevaert, Ileana Ghiordanescu, Peter Hellings, Karin Hoffmann-Sommergruber, Ayse Fusun Kalpakioglu, Farid Marmouz, Ángela Meijide Calderón, Ralph Mösges, Alla Nakonechna, Markus Ollert, José Oteros, Giovanni Pajno, Catalina Panaitescu, Daniel Perez-Formigo, Oliver Pfaar, Constantinos Pitsios, Michael Rudenko, Dermot Ryan, Silvia Sánchez-García, Jennifer Shih, Salvatore Tripodi, Lauri-Ann Van der Poel, Harmieke van Os-Medendorp, Gilda Varricchi, Jörn Wittmann, Margitta Worm, Ioana Agache

Allergy, 75(2):259-272 (2020)

IL-4 receptor α blockade prevents sensitization and alters acute and long-lasting effects of allergen-specific immunotherapy of murine allergic asthma

Dennis Russkamp*, Antonio Aguilar-Pimentel*, Francesca Alessandrini, Valérie Gailus-Durner, Helmut Fuchs, Caspar Ohnmacht, Adam Chaker, Martin Hrabe de Angelis, Markus Ollert, Carsten B. Schmidt-Weber, Simon Blank

Allergy, 74(8):1549-1560 (2019)

RelB deficiency in dendritic cells protects from autoimmune inflammation due to spontaneous accumulation of tissue T regulatory cells

Nico Andreas*, Maria Potthast*, Anna-Lena Geiselhöringer*, Garima Garg, Renske de Jong, Julia Riewaldt, Dennis Russkamp, Marc Riemann, Jean-Philippe Girard, Simon Blank, Karsten Kretschmer, Carsten Schmidt-Weber, Thomas Korn, Falk Weih, Caspar Ohnmacht

Journal of Immunology, 203(10):2602-2613 (2019)

The role of component-resolved diagnosis in Hymenoptera venom allergy

Maria Beatrice Bilò, Markus Ollert, Simon Blank

Current Opinion in Allergy and Clinical Immunology, 19(6):614-622 (2019)

Supporting allergen-specific immunotherapy by inhibition of Janus kinases 

Jan Gutermuth, Carsten B Schmidt-Weber, Simon Blank

Allergy, 74(9):1814-1816 (2019)

Insektengiftallergie: Komponentenaufgelöste Diagnostik und ihre Implikationen für die Therapie

Simon Blank

Haut, 01/19:8-13 (2019)

EAACI position paper: Comparing insect hypersensitivity induced by bite, sting, inhalation or ingestion in human beings and animals

Isabella Pali-Schöll, Simon Blank, Kitty Verhoeckx, Ralf S Mueller, Jozef Janda, Eliane Marti, Ahmed A Seida, Claudio Rhyner, Douglas J DeBoer, Erika Jensen-Jarolim

Allergy, 74(5):874-887 (2019)

Prevalence of Hymenoptera venom allergy and sensitization in the population-representative German KORA cohort

Simon Blank*, Stephanie Haemmerle*, Teresa Jaeger, Dennis Russkamp, Johannes Ring, Carsten B. Schmidt-Weber, Markus Ollert

Allergo Journal International, 28(6):183-91 (2019)

From basic science to diagnostics - History of a patent on a honeybee venom allergen

Simon Blank

Allergy, 74(2): 385-387 (2019)

Basophil activation test differentiates between patients with alpha-Gal-syndrome and asymptomatic alpha-Gal sensitization

Jana Mehlich, Jörg Fischer, Christiane Hilger, Martine Morriset, Francoise Codreanu-Morel, Maximilian Schiener, Simon Blank, Markus Ollert, Ulf Darsow, Tilo Biedermann, Bernadette Eberlein

Journal of Allergy and Clinical Immunology, 143(1):182-189 (2019)

Characterization of the honeybee venom proteins C1q-like protein and PVF1 and their allergenic potential

Dennis Russkamp*, Matthias Van Vaerenbergh*, Stefanie Etzold, Bernadette Eberlein, Ulf Darsow, Maximilian Schiener, Lina De Smet, Magdalena Absmaier, Tilo Biedermann, Edzard Spillner, Markus Ollert, Thilo Jakob, Carsten B. Schmidt-Weber, Dirk C. de Graaf, Simon Blank

Toxicon, 150:198-206 (2018)

Component-resolved diagnostics to direct in venom immunotherapy: important steps towards precision medicine

Simon Blank, Maria Beatrice Bilò, Markus Ollert

Clinical and Experimental Alergy, 48(4):354-364 (2018)

The high molecular weight dipeptidyl peptidase IV Pol d 3 is a major allergen of Polistes dominula venom

Maximilian Schiener, Christiane Hilger, Bernadette Eberlein, Mariona Pascal, Annette Kuehn, Dominique Revets, Sébastien Planchon, Gunilla Pietsch, Pilar Serrano, Carmen Moreno-Aguilar, Federico de la Roca, Tilo Biedermann, Ulf Darsow, Carsten B. Schmidt-Weber, Markus Ollert*, Simon Blank*

Scientific Reports, 8(1):1318 (2018)

Allergien durch Stiche und Bisse von Insekten und Spinnentieren.

Simon Blank, Maximilian Schiener, Markus Ollert

In: Tierallergien. ed. Annette Kühn; Christiane Hilger. Dustri Verlag (2017)

Improved efficacy of allergen-specific immunotherapy by JAK inhibition in a murine model of allergic asthma

Antonio Aguilar-Pimentel*, Anke Graessel*, Francesca Alessandrini, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabě de Angelis, Dennis Russkamp, Adam Chaker, Markus Ollert, Simon Blank*, Jan Gutermuth*, Carsten B. Schmidt-Weber*

PLOS ONE, 12(6): e0178563 (2017)

Allergen-specific immunotherapy of Hymenoptera venom allergy - also a matter of diagnosis

Maximilian Schiener, Anke Graessel, Markus Ollert, Carsten B. Schmidt-Weber, Simon Blank

Human Vaccines and Immunotherapeutics, 13(10): 2467-2481 (2017)

Component-resolved evaluation of the content of major allergens in therapeutic extracts for specific immunotherapy of honeybee venom allergy

Simon Blank, Stefanie Etzold, Ulf Darsow, Maximilian Schiener, Bernadette Eberlein, Dennis Russkamp, Sara Wolf, Anke Graessel, Tilo Biedermann, Markus Ollert, Carsten B. Schmidt-Weber

Human Vaccines and Immunotherapeutics, 13(10): 2482-2489 (2017)

Benefits and limitations of recombinant allergens in diagnostics of insect venom allergy

Edzard Spillner, Simon Blank, Thilo Jakob

In: Molecular Allergy Diagnostics. ed. Thilo Jakob; Jörg Kleine-Tebbe. Springer Verlag (2017)

Application of recombinant antigen 5 allergens from 7 allergy-relevant Hymenoptera species in diagnostics

Maximilian Schiener, Bernadette Eberlein, Carmen Moreno-Aguilar, Gunilla Pietsch, Pilar Serrano, Mareike McIntyre, Lea Schwarze, Dennis Russkamp, Tilo Biedermann, Edzard Spillner, Ulf Darsow, Markus Ollert*, Carsten B. Schmidt-Weber, Simon Blank* 

Allergy,72(1): 98-108 (2017)

Komponenten-aufgelöste Diagnostik der Hymenopterengiftallergie

Anke Graessel, Maximilian Schiener, Stefanie Etzold, Dennis Russkamp, Simon Blank

BIOspektrum, 22(7): 713-716 (2016)

EAACI Molecular Allergology Users’s Guide

Paolo M Matricardi, Jörg Kleine-Tebbe, Hans Jürgen Hoffmann, Rudolf Valenta, Christiane Hilger, Stephanie Hofmaier, Rob C Aalberse, Iona Agache, Riccardo Asero, Barbara Ballmer-Weber, Domingo Barber, Kirsten Beyer, Tilo Biedermann, Maria Beatrice Bilo, Simon Blank, Barbara Bohle, Philipp P. Bosshard, Heimo Breiteneder, Helen A Brough, Luis Caraballo, Jean-Christoph Caubet, Reto Crameri, Janet M Davies, Nikos Douladiris, Motohiro Ebisawa, Philippe A Eigenmann, Montserrat Fernandez-Rivas, Fatima Ferreira, Gabriele Gadermaier, Martin Glatz, Robert G. Hamilton, Thomas Hawranek, Peter Hellings, Karin Hoffmann-Sommergruber, Thilo Jakob, Uta Jappe, Marek Jutel, Sandip D Kamath, Edward F Knol, Peter Korosec, Annette Kuehn, Gideon Lack, Andreas L Lopata, Mika Mäkelä, Martine Morisset, Verena Niederberger, Anna H Nowak-Wezgrzyn, Nikolaos G Papadopoulos, Elide A Pastorello, Gabrielle Pauli, Thomas Platts-Mills, Lars K Poulsen, Monika Raulf, Joaquin Sastre, Enrico Scala, Johannes M Schmid, Peter Schmid-Grendelmeier, Marianne van Hage, Ronald van Ree, Stefan Vieths, Richard Weber, Magnus Wickman, Antonella Muraro, Markus Ollert

Pediatric Allergy and Immunology, 27 (Suppl. 1): 1-236 (2016)

Allergien durch Stiche und Bisse von Insekten und Spinnentieren

Simon Blank, Maximilian Schiener, Markus Ollert

Allergologie, 39(6): 265-273 (2016)

Added sensitivity of component-resolved diagnosis in hymenoptera venom-allergic patients with elevated serum tryptase and/or mastocytosis

Jana Michel, Knut Brockow, Ulf Darsow, Johannes Ring, Carsten Schmidt-Weber, Thomas Grunwald, Simon Blank*, Markus Ollert*

Allergy, 71(5): 651-660 (2016)

Molekulare Ansätze zur Optimierung diagnostischer Konzepte und therapeutischer Interventionen in der Allergologie

Simon Blank

Gutachter: Prof. Dr. Carsten Schmidt-Weber, Prof. Dr. Markus Ollert, Prof. Dr. Thilo Biedermann

Habilitationsschrift, München (2016)

Mehrwert und Grenzen rekombinanter Allergene in der Diagnostik der Insektengiftallergie

Edzard Spillner, Simon Blank, Thilo Jakob

In: Molekulare Allergologie. ed. / Thilo Jakob; Jörg Kleine-Tebbe. Springer Verlag (2015)

Anaphylaxis to insect venom allergens: Role of molecular diagnostics

Markus Ollert* and Simon Blank*

Current Allergy and Asthma Reports, 15:26 (2015)

Decline of Ves v 5-specific blocking capacity in wasp venom-allergic patients after stopping allergen immunotherapy

Christian Möbs, Jan Müller, Annika Rudzio, Julia Pickert, Simon Blank, Thilo Jakob, Edzard Spillner, Wolfgang Pfützner

Allergy, 70(6): 715-719 (2015)

IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology

Matthias van Vaerenbergh, Lina de Smet, David Rafei-Shamsabadi, Simon Blank, Edzard Spillner, Didier Ebo, Bart Devreese, Thilo Jakob, Dirk C. de Graaf

Molecular Immunology, 63(2): 449-455 (2015)

Basophil activation test using recombinant allergens: highly specific diagnostic method complementing routine tests in wasp venom allergy

Lukas Balzer, Davide Pennino, Simon Blank, Henning Seismann, Ulf Darsow, Mathias Schnedler, Mareike McIntyre, Markus Ollert, Steven R. Durham, Edzard Spillner, Johannes Ring, and Liliana Cifuentes

PLOS ONE, 9(10):e108619 (2014).

Reply to: Recombinant allergens rarely allow identification of Hymenoptera venom-allergic patients with negative specific IgE to whole venom preparations

Liliana Cifuentes, Simon Blank, Davide Pennino, Jana Michel, Ulf Darsow, Johannes Ring, and Markus Ollert

Journal of Allergy and Clinical Immunology, 134(2): 494-495 (2014)

Hymenoptera Allergens: From Venom to “Venome”

Edzard Spillner, Simon Blank, Thilo Jakob

Frontiers in Immunology, 5:77 (2014)

Component resolution reveals additional major allergens in patients with honeybee venom allergy

Julian Köhler*, Simon Blank*, Sabine Müller, Frank Bantleon, Marcel Frick, Johannes Huss-Marp, Jonas Lidholm, Edzard Spillner, and Thilo Jakob

Journal of Allergy and Clinical Immunology, 133 (5): 1383-1389 (2014)

Identification of hymenoptera venom-allergic patients with negative specific IgE to venom extract by using recombinant allergens

Liliana Cifuentes, Sebastian Vosseler, Simon Blank, Henning Seismann, Thomas Grunwald, Ulf Darsow, Reinhard Bredehorst, Martin Mempel, Edzard Spillner, Johannes Ring, and

Markus Ollert

Journal of Allergy and Clinical Immunology, 133 (3): 909-910 (2014)

Donor assists acceptor binding and catalysis of human alpha1,6-fucosyltransferase

Miriam P. Kötzler, Simon Blank, Frank I. Bantleon, Martin Wienke, Edzard Spillner, and Bernd Meyer

ACS Chemical Biology, 8 (8): 1830-1840 (2013)

Vitellogenins are new high molecular weight components and allergens (Api m 12 and Ves v 6) of Apis mellifera and Vespula vulgaris venom

Simon Blank, Henning Seismann, Mareike McIntyre, Markus Ollert, Frank I. Bantleon, and Edzard Spillner

PLOS ONE, 8(4):e62009 (2013)

Polistes species venom is devoid of carbohydrate-based cross-reactivity and allows interference free diagnostics

Simon Blank, Charles Neu, Daniel Hasche, Frank I. Bantleon, Thilo Jakob, and Edzard Spillner

Journal of Allergy and Clinical Immunology, 131 (4): 1239-1242 (2013)

Extending the honeybee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5

Matthias van Vaerenbergh, Dries Cardoen, Ellen Formesyn, Marleen Brunain, Gonzalez van Driessche, Simon Blank, Edzard Spillner, Peter Verleyen, Tom  Wenseleers, Liliane Schoofs, Bart Devreese, and Dirk de Graaf

Insect Molecular Biology, 22 (2): 199-210 (2013)

An IgE epitope of Bet v 1 and fagales PR10 proteins as defined by a human monoclonal IgE

Julia Hecker, Andrea Diethers, Daniela Schulz, Amir Sabri, Melanie Plum, Yvonne Michel, Martin Mempel, Markus Ollert, Thilo Jakob, Simon Blank, Ingke Braren, and Edzard Spillner

Allergy, 67 (12): 1530-1537 (2012)

Donor substrate binding and enzymatic mechanism of human core alpha1,6-fucosyltransferase (FUT8)

Miriam P. Kötzler, Simon Blank, Frank I. Bantleon, Edzard Spillner, and Bernd Meyer

Biochim Biophys Acta, 1820 (12): 1915-1925 (2012)

Avian IgY antibodies and their recombinant equivalents in research, diagnostics and therapy

Edzard Spillner, Ingke Braren, Kerstin Greunke, Henning Seismann, Simon Blank, Dion du Plessis

Biologicals, 40 (5): 313-322 (2012)

The putative serine protease inhibitor allergen Api m 6 from A. mellifera venom: recombinant and structural evaluation

YvonneMichel, Mareike McIntyre, Helene Ginglinger, Markus Ollert, Liliana Cifuentes, Simon Blank*, and Edzard Spillner*

J Investig Allergol Clin Immunol, 22 (7): 476-484 (2012)

Perspectives, pitfalls and current status of molecular diagnosis in insect venom allergy

Edzard Spillner, Simon Blank, and Thilo Jakob

Allergo Journal International, 21 (4): 249-256 (2012)

Recombinant IgE antibody engineering to target EGFR

Edzard Spillner, Melanie Plum, Simon Blank, Michaela Miehe, Josef Singer, and Ingke Braren

Cancer Immunology and Immunotherapy, 61 (9): 1565-1573 (2012)

Comparable IgE reactivity to natural and recombinant Api m 1 in cross-reactive carbohydrate determinant-negative patients with bee venom allergy

Thilo Jakob, Julian Köhler, Simon Blank, Ulrika Magnusson, Johannes Huss-Marp, Edzard Spillner, and Jonas Lidholm

Journal of Allergy and Clinical Immunology, 130 (7): 276-278 (2012)

The major royal jelly proteins 8 and 9 (Api m 11) are glycosylated components of Apis mellifera venom with allergenic potential beyond carbohydrate based reactivity

Simon Blank, Frank I. Bantleon, Mareike McIntyre, Markus Ollert, and Edzard Spillner

Clinical and Experimental Allergy,42 (6): 976-985 (2012)

Close-up of the alpha-Gal epitope as defined by a monoclonal chimeric IgE and human sera using saturation transfer difference

Melanie Plum, Yvonne Michel, Kathatrina Wallach, Tim Raiber, Simon Blank, Frank Bantleon, Andrea Diethers, Kerstin Greunke, Ingke Braren and Thomas Hackl, Bernd Meyer, and Edzard Spillner

Journal of Biological Chemistry, 286 (50): 43103-43111 (2011)

Formation of the immunogenic α1,3-fucose epitope: Elucidation of substrate specificity and of enzyme mechanism of core fucosyltransferase A

Miriam P. Kötzler*, Simon Blank*, Henning N. Behnken, Dirk Alpers, Frank I. Bantleon, Edzard Spillner, and Bernd Meyer

Insect Biochemistry and Molecular Biology, 42 (2): 116-125 (2011)

Rekombinante Insektengiftallergene: Ist das Bienen- oder doch das Wespengift schuld?

Thilo Jakob, Simon Blank, Edzard Spillner, and Markus Ollert

Der Deutsche Dermatologe, 59: 759-761 (2011)

Stress-resistant neural stem cells positively influence regional energy metabolism after spinal cord injury in mice

Karsten Schwerdtfeger, Angelika E.M. Mautes, Christian Bernreuther, Yifang Cui, Jérôme Manville, Marcel Dihné, Simon Blank, and Melitta Schachner

Journal of Molecular Neuroscience, 46 (2): 401-409 (2011)

Api m 10, a genuine A. mellifera venom allergen, is clinically relevant but underrepresented in therapeutic extracts

Simon Blank, Henning Seismann, Yvonne Michel, Mareike McIntyre, Ingke Braren, Thomas Grunwald, Ulf Darsow, Johannes Ring, Reinhard Bredehorst Markus Ollert, and Edzard Spillner

Allergy,66 (10): 1322-1329 (2011)

Generation and epitope analysis of human monoclonal antibody isotypes with specificity for the timothy grass major allergen Phl p 5a

Julia Hecker, Andrea Diethers, Stefanie Etzold, Henning Seismann, Yvonne Michel, Melanie Plum, Reinhard Bredehorst, Simon Blank, Ingke Braren*, and Edzard Spillner*

Molecular Immunology, 48 (9-10):1236-1244 (2011)

Evaluation of different glycoforms of honeybee venom major allergen phospholipase A2 (Api m 1) produced in insect cells

Simon Blank*, Yvonne Michel*, Henning Seismann, Melanie Plum, Kerstin Greunke Thomas Grunwald, Reinhard Bredehorst, Markus Ollert, Ingke Braren, and Edzard Spillner

Protein and Peptide Letters, 18 (4): 415-422 (2011)

Reply to: Detection of IgE to recombinant Api m 1 and rVes v 5 is valuable but not sufficient to distinguish bee from wasp venom allergy

Silke Hofmann, Nikolai Pfender, Steffi Weckesser, Simon Blank, Johannes Huss-Marp, Edzard Spillner, and Thilo Jakob

Journal of Allergy and Clinical Immunology, 128 (1): 248 (2011)

Detection of IgE to recombinant Api m 1 and rVes v 5 is valuable but not sufficient to distinguish bee from wasp venom allergy

Gunter J. Sturm, Wolfgang Hemmer, Thomas Hawranek, Roland Lang, Markus Ollert, Edzard Spillner Simon Blank, Danijela Bokanovic, and Werner Aberer

Journal of Allergy and Clinical Immunology, 128 (1): 247-248 (2011)

Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity

HenningSeismann, Simon Blank, Liliana Cifuentes, Ingke Braren, Reinhard Bredehorst, Thomas Grunwald, Markus Ollert, and Edzard Spillner

Clinical and Molecular Allergy, 8: 7 (2010)

Identification, recom­binant expression and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3

Simon Blank*, Henning Seismann*, Benjamin Bockisch*, Ingke Braren, Liliana Cifuentes, Mareike McIntyre, Dana Rühl, Johannes Ring, Reinhard Bredehorst, Markus Ollert, Thomas Grunwald, and Edzard Spillner

Journal of Immunology, 184 (9): 5403-5413 (2010)

Components and mechanisms in diagnosis and therapy of hymenoptera venom allergy

Simon Blank

Gutachter: Prof. Dr. Reinhard Bredehorst, Prof. Dr. Bernd Meyer, Prof. Dr. Martina Kerscher, Dr. Thomas Hackl

Dissertation, Hamburg (2009)

Dissecting cross-reactivity in hymenop­tera venom allergy by circumvention of alpha-1,3 core fucosylation

Henning Seismann*, Simon Blank*, Ingke Braren, Kerstin Greunke, Liliana Cifuentes, Thomas Grunwald, Reinhard Bredehorst, Markus Ollert, and Edzard Spillner

Molecular Immunology, 47(4): 799-808 (2010)

Recombinant IgY for improvement of immunoglobulin-based analytical appli­cations

Kerstin Greunke, Ingke Braren, Iris Alpers, Simon Blank, Jan Sodenkamp, Reinhard Bredehorst, and Edzard Spillner

Clinical Biochemistry, 41:1237-1244 (2008)

Generation of human monoclonal allergen-specific IgE and IgG anti­bodies from synthetic antibody libraries

IngkeBraren, Simon Blank, Henning Seismann, Susanne Deckers, Markus Ollert, Thomas Grunwald, and Edzard Spillner

Clinical Chemistry, 53(5): 837-844 (2007)

*equal contribution as first or senior author